Cpac 3 write up Make a temporary squash preparation of a root tip to show stages of mitosis in the meristem under the light microscope Method:
Even though this experiment was carried out with a high degree of accuracy, we as a sample size were not able to calculate any mitotic index, due to a number of reasons, one being the strength of the microscopes was not great enough to properly see the stages of mitosis or the fact that there was experimental error which shows that the experiment is very sensitive, as a suggestion I would use a different sample due to the cumbersome process of maceration and I would also txt to improve the quality of the microscopes in both their resolution, to increase image sharpness and magnification to decrease the size of the frame of reference.
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Core practical 5 write up “Investigate the effect of temperature on beetroot membrane permeability” Method:
For safety we had the technician cut us the cores and wore safety goggles.
In conclusion I can see a negative correlation between temperature and average transmission, and I conclude that these results can be interpreted correctly because the results were not compromised by any experimental error and this is shown by the absence of any anomalous results. These results were backed up by this study[1], which showed that temperature has a negative correlation with membrane permeability, in wheat, which shows that our results can be reciprocated in and among the scientific community. I feel that the experiment could be improved by having more data points which would give us a more reliable result and it would help us. [1] Blum, A. and Ebercon, A., 1981. Cell membrane stability as a measure of drought and heat tolerance in wheat. Crop Science, 21(1), pp.43-47. Core practical 2 write up “Use of the light microscope, including simple stage and eyepiece micrometres and drawing small numbers of cells from a specialised tissue” Method:
Results: Evaluation:
In my view this experiment could be repeated with accuracy and reliability, which would enable to observe samples in greater detail and to more fully understand the infrastructure of the microscopic world. |
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